Aerobic exercise intensity does not affect the anabolic signaling following resistance exercise in endurance athletes

Sci Rep. 2021 May 24;11(1):10785. doi: 10.1038/s41598-021-90274-8.


This study examined whether intensity of endurance stimulus within a concurrent training paradigm influenced the phosphorylation of signaling proteins associated with the mTOR and AMPK networks. Eight male cyclists completed (1) resistance exercise (RES), 6 × 8 squats at 80% 1-RM; (2) resistance exercise and moderate intensity cycling of 40 min at 65% V̇O2peak, (RES + MIC); (3) resistance exercise and high intensity interval cycling of 40 min with 6 alternating 3 min intervals of 85 and 45% V̇O2peak (RES + HIIC), in a cross-over design. Muscle biopsies were collected at rest and 3 h post-RES. There was a main effect of condition for mTORS2448 (p = 0.043), with a greater response in the RES + MIC relative to RES condition (p = 0.033). There was a main effect of condition for AMPKα2T172 (p = 0.041), with a greater response in RES + MIC, relative to both RES + HIIC (p = 0.026) and RES (p = 0.046). There were no other condition effects for the remaining protein kinases assessed (p > 0.05). These data do not support a molecular interference effect in cyclists under controlled conditions. There was no intensity-dependent regulation of AMPK, nor differential activation of anabolism with the manipulation of endurance exercise intensity.

Publication types

  • Comparative Study

MeSH terms

  • AMP-Activated Protein Kinases / metabolism*
  • Adult
  • Cross-Over Studies
  • Endurance Training / methods*
  • Exercise / physiology*
  • Humans
  • Male
  • Muscle, Skeletal / metabolism
  • Phosphorylation
  • Resistance Training / methods*
  • Signal Transduction
  • TOR Serine-Threonine Kinases / metabolism*


  • MTOR protein, human
  • PRKAA2 protein, human
  • TOR Serine-Threonine Kinases
  • AMP-Activated Protein Kinases