Quantitative analyses of CD7, CD33, CD34, CD56, and CD123 within the FLT3-ITD/ NPM1-MUT myeloblastic/monocytic bulk AML blastic populations

Leuk Lymphoma. 2021 Nov;62(11):2716-2726. doi: 10.1080/10428194.2021.1927018. Epub 2021 May 26.


The most frequent mutations in acute myeloid leukemia (AML) - FLT3-ITD and NPM1 - are associated with a specific immunophenotype. We evaluated the levels of surface antigens in an uninvestigated AML patient population according to the combination of FLT3-ITD/NPM1 mutations. Antigen levels were calculated as the geometric mean fluorescence index (MFI) ratio between myeloblasts or monoblasts/monocytes and a negative population for the specific antigen. In myeloblastic populations, FLT3-ITD cases presented CD7high MFI values (p < .001), while NPM1-MUT cases presented CD33high (p < .001), and CD34low (p < .001) MFI values. Within the monoblastic/monocytic populations, CD56high expression was observed only in the FLT3-WT/NPM1-MUT population (p=.003). The single common antigen expression between myeloblasts and monoblasts/monocytes was CD123high expression only within the FLT3-ITD/NPM1-MUT subgroup. Our results present a subtle influence of FLT3-ITD/NPM1 mutations upon antigen expression profiles in myeloblasts vs monoblasts/monocytes, and we described a novel correlation between the presence of NPM1 and CD56high values within bulk leukemic monoblasts/monocytes.

Keywords: AML; FLT3-ITD; MFI; NPM1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Granulocyte Precursor Cells
  • Humans
  • Interleukin-3 Receptor alpha Subunit*
  • Leukemia, Myeloid, Acute* / diagnosis
  • Leukemia, Myeloid, Acute* / genetics
  • Leukocyte Count
  • Monocytes
  • Mutation
  • Nuclear Proteins / genetics
  • Nucleophosmin
  • Prognosis
  • Sialic Acid Binding Ig-like Lectin 3
  • fms-Like Tyrosine Kinase 3 / genetics


  • CD33 protein, human
  • Interleukin-3 Receptor alpha Subunit
  • NPM1 protein, human
  • Nuclear Proteins
  • Sialic Acid Binding Ig-like Lectin 3
  • Nucleophosmin
  • FLT3 protein, human
  • fms-Like Tyrosine Kinase 3