An in vitro human muscle preparation suitable for metabolic studies. Decreased insulin stimulation of glucose transport in muscle from morbidly obese and diabetic subjects

J Clin Invest. 1988 Aug;82(2):486-94. doi: 10.1172/JCI113622.


We have developed an in vitro muscle preparation suitable for metabolic studies with human muscle tissue and have investigated the effects of obesity and non-insulin-dependent diabetes mellitus (NIDDM) on glucose transport. Transport of 3-O-methylglucose and 2-deoxyglucose was stimulated approximately twofold by insulin in muscle from normal nonobese subjects and stimulation occurred in the normal physiological range of insulin concentrations. In contrast to insulin stimulation of 3-O-methylglucose and 2-deoxyglucose transport in muscle from normal, nonobese subjects, tissue from morbidly obese subjects, with or without NIDDM, were not responsive to insulin. Maximal 3-O-methylglucose transport was lower in muscle of obese than nonobese subjects. Morbidly obese patients, with or without NIDDM, have a severe state of insulin resistance in glucose transport. The novel in vitro human skeletal muscle preparation herein described should be useful in investigating the mechanism of this insulin resistance.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3-O-Methylglucose
  • Adult
  • Biological Transport / drug effects
  • Deoxyglucose / metabolism
  • Diabetes Mellitus, Type 2 / complications
  • Diabetes Mellitus, Type 2 / metabolism*
  • Diabetes Mellitus, Type 2 / pathology
  • Female
  • Glucose / metabolism*
  • Glucose Tolerance Test
  • Humans
  • In Vitro Techniques
  • Insulin Resistance*
  • Male
  • Methylglucosides / metabolism
  • Muscles / metabolism*
  • Muscles / ultrastructure
  • Obesity, Morbid / complications
  • Obesity, Morbid / metabolism*
  • Obesity, Morbid / pathology
  • Time Factors


  • Methylglucosides
  • 3-O-Methylglucose
  • Deoxyglucose
  • Glucose