Sucrase-isomaltase deficiency in humans. Different mutations disrupt intracellular transport, processing, and function of an intestinal brush border enzyme

J Clin Invest. 1988 Aug;82(2):667-79. doi: 10.1172/JCI113646.


Eight cases of congenital sucrase-isomaltase deficiency were studied at the subcellular and protein level with monoclonal antibodies against sucrase-isomaltase. At least three phenotypes were revealed: one in which sucrase-isomaltase protein accumulated intracellularly probably in the endoplasmic reticulum, as a membrane-associated high-mannose precursor, one in which the intracellular transport of the enzyme was apparently blocked in the Golgi apparatus, and one in which catalytically altered enzyme was transported to the cell surface. All patients expressed electrophoretically normal or near normal high-mannose sucrase-isomaltase. The results suggest that different, probably small, mutations in the sucrase-isomaltase gene lead to the synthesis of transport-incompetent or functionally altered enzyme which results in congenital sucrose intolerance.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Biological Transport
  • Body Fluids / enzymology*
  • Child
  • Female
  • Humans
  • Immunohistochemistry
  • Infant
  • Intestinal Mucosa / enzymology*
  • Intestinal Mucosa / metabolism
  • Intestinal Mucosa / physiology
  • Intracellular Fluid / enzymology*
  • Intracellular Fluid / metabolism
  • Intracellular Fluid / physiology
  • Male
  • Microvilli / enzymology*
  • Microvilli / metabolism
  • Microvilli / physiology
  • Multienzyme Complexes / deficiency*
  • Mutation*
  • Phenotype
  • RNA Processing, Post-Transcriptional*
  • Sucrase-Isomaltase Complex / deficiency*
  • Sucrase-Isomaltase Complex / isolation & purification
  • Sucrase-Isomaltase Complex / physiology
  • Sucrose / administration & dosage
  • Syndrome
  • Twins, Monozygotic


  • Multienzyme Complexes
  • Sucrose
  • Sucrase-Isomaltase Complex