Isolation of genetically manipulated neural progenitors and immature neurons from embryonic mouse neocortex by FACS

STAR Protoc. 2021 May 17;2(2):100540. doi: 10.1016/j.xpro.2021.100540. eCollection 2021 Jun 18.


The embryonic mammalian neocortex includes neural progenitors and neurons at various stages of differentiation. The regulatory mechanisms underlying multiple aspects of neocortical development-including cell division, neuronal fate commitment, neuronal migration, and neuronal differentiation-have been explored using in utero electroporation and virus infection. Here, we describe a protocol for investigation of the effects of genetic manipulation on neural development through direct isolation of neural progenitors and neurons from the mouse embryonic neocortex by fluorescence-activated cell sorting. For complete details on the use and execution of this protocol, please refer to Tsuboi et al. (2018) and Sakai et al. (2019).

Keywords: Cell Biology; Cell Differentiation; Cell isolation; Developmental biology; Flow Cytometry/Mass Cytometry; Gene Expression; Molecular Biology; Neuroscience; Stem Cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Embryo, Mammalian / cytology
  • Flow Cytometry / methods*
  • Mice
  • Mice, Transgenic
  • Neocortex* / cytology
  • Neocortex* / embryology
  • Neural Stem Cells / cytology*
  • Neurons / cytology