Isolation of genetically manipulated neural progenitors and immature neurons from embryonic mouse neocortex by FACS

Yusuke Kishi; Yukiko Gotoh

doi:10.1016/j.xpro.2021.100540

Isolation of genetically manipulated neural progenitors and immature neurons from embryonic mouse neocortex by FACS

STAR Protoc. 2021 May 17;2(2):100540. doi: 10.1016/j.xpro.2021.100540. eCollection 2021 Jun 18.

Authors

Yusuke Kishi¹, Yukiko Gotoh^{1

2}

Affiliations

¹ Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
² International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Abstract

The embryonic mammalian neocortex includes neural progenitors and neurons at various stages of differentiation. The regulatory mechanisms underlying multiple aspects of neocortical development-including cell division, neuronal fate commitment, neuronal migration, and neuronal differentiation-have been explored using in utero electroporation and virus infection. Here, we describe a protocol for investigation of the effects of genetic manipulation on neural development through direct isolation of neural progenitors and neurons from the mouse embryonic neocortex by fluorescence-activated cell sorting. For complete details on the use and execution of this protocol, please refer to Tsuboi et al. (2018) and Sakai et al. (2019).

Keywords: Cell Biology; Cell Differentiation; Cell isolation; Developmental biology; Flow Cytometry/Mass Cytometry; Gene Expression; Molecular Biology; Neuroscience; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Embryo, Mammalian / cytology
Flow Cytometry / methods*
Mice
Mice, Transgenic
Neocortex* / cytology
Neocortex* / embryology
Neural Stem Cells / cytology*
Neurons / cytology