A limited panel of INSM1 and LEF1 immunostains accurately distinguishes between pancreatic neuroendocrine tumor and solid pseudopapillary neoplasm

Pathol Res Pract. 2021 Jul:223:153462. doi: 10.1016/j.prp.2021.153462. Epub 2021 May 13.

Abstract

Solid pseudopapillary neoplasm (SPN) and well differentiated pancreatic neuroendocrine tumor (PNET) can show significant cytomorphological overlap. In this study, we evaluated the role of INSM1 and LEF1 immunohistochemical stains in distinguishing between these two tumors. 22 SPN and 25 PNET surgically resected cases were stained for both INSM1 and LEF1. All the 22 cases of SPN showed strong and diffuse nuclear staining for LEF1 (in >95 % of tumor cells), while all 25 PNET were negative for LEF1. All 25 PNET cases were positive for INSM1 (moderate to strong intensity nuclear staining in >50 % of the tumor cells), while all 22 cases of SPN were negative for INSM1. The results of our study show that a limited panel comprising of INSM1 and LEF1 immunostains accurately distinguishes between SPN and PNET.

Keywords: INSM1; LEF1; Neuroendocrine; Pancreas; Solid pseudopapillary.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / analysis*
  • Child
  • Diagnosis, Differential
  • Female
  • Humans
  • Immunohistochemistry*
  • Lymphoid Enhancer-Binding Factor 1 / analysis*
  • Male
  • Middle Aged
  • Neuroendocrine Tumors / chemistry*
  • Neuroendocrine Tumors / pathology
  • Pancreatic Neoplasms / chemistry*
  • Pancreatic Neoplasms / pathology
  • Predictive Value of Tests
  • Repressor Proteins / analysis*
  • Young Adult

Substances

  • Biomarkers, Tumor
  • LEF1 protein, human
  • Lymphoid Enhancer-Binding Factor 1
  • Repressor Proteins
  • INSM1 protein, human