Effects of Moringa Extract on Aminoglycoside-Induced Hair Cell Death and Organ of Corti Damage

Otol Neurotol. 2021 Sep 1;42(8):1261-1268. doi: 10.1097/MAO.0000000000003193.


Hypothesis: Moringa extract, a naturally occurring anti-oxidant, protects against aminoglycoside-induced hair cell death and hearing loss within the organ of Corti.

Background: Reactive oxygen species (ROS) arise primarily in the mitochondria and have been implicated in aminoglycoside-induced ototoxicity. Mitochondrial dysfunction results in loss of membrane potential, release of caspases, and cell apoptosis. Moringa extract has not previously been examined as a protective agent for aminoglycoside-induced ototoxicity.

Methods: Putative otoprotective effects of moringa extract were investigated in an organotypic model using murine organ of Corti explants subjected to gentamicin-induced ototoxicity. Assays evaluated hair cell loss, cytochrome oxidase expression, mitochondrial membrane potential integrity, and caspase activity.

Results: In vitro application of moringa conferred significant protection from gentamicin-induced hair cell loss at dosages from 25 to 300 μg/mL, with dosages above 100 μg/mL conferring near complete protection. Assays demonstrated moringa extract suppression of ROS, preservation of cytochrome oxidase activity, and reduction in caspase production.

Conclusion: Moringa extract demonstrated potent antioxidant properties with significant protection against gentamicin ototoxicity in cochlear explants.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Aminoglycosides* / toxicity
  • Animals
  • Apoptosis
  • Cell Death
  • Gentamicins / toxicity
  • Hair Cells, Auditory
  • Mice
  • Moringa*
  • Organ of Corti
  • Plant Extracts / pharmacology


  • Aminoglycosides
  • Gentamicins
  • Plant Extracts