In Vivo and In Vitro Models for the Study of Bone Remodeling and the Role of Immune Cells

Methods Mol Biol. 2021:2325:97-106. doi: 10.1007/978-1-0716-1507-2_7.

Abstract

Immune and bone cells cross talk has been established by different years; however the underlying mechanisms require continuous investigation. To this end both in vivo and in vitro models have been realized and some of this are described in this chapter. In particular, here we described the animal models used for the understanding of lymphocyte role in bone homeostasis, together with some in vitro models.

Keywords: Animal models; B cells; Bone cells; Osteoblasts; Osteoclasts; Osteoimmunology; T-cells.

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • B-Lymphocytes / immunology*
  • Bone Remodeling*
  • Cell Line
  • Collagen Type I / metabolism
  • Homeostasis
  • Humans
  • Mice
  • Osteocalcin / metabolism
  • Osteoclasts / metabolism*
  • Osteocytes / metabolism*
  • Osteogenesis*
  • T-Lymphocytes / immunology*
  • Tartrate-Resistant Acid Phosphatase / metabolism
  • Tomography, X-Ray Computed / methods

Substances

  • Collagen Type I
  • Osteocalcin
  • Alkaline Phosphatase
  • Tartrate-Resistant Acid Phosphatase