Lipase immobilization on biodegradable film with sericin

Biotechnol Appl Biochem. 2022 Apr;69(2):660-667. doi: 10.1002/bab.2141. Epub 2021 Jun 7.

Abstract

An ecofriendly and low-cost film composed by cassava starch, polyvinyl alcohol, and sericin blend (CS-PVA-SS) was synthesized, characterized, and applied as a novel support for Botryosphaeria ribis EC-01 lipase immobilization by enzyme-film-enzyme adsorption. Film revealed thickness between 230 and 309 μm and higher flexibility and malleability in comparison with film without SS. Based on p-nitrophenyl palmitate hydrolysis reaction, the activity retention of immobilized lipase was 987%. For optimal conditions, the yield in ethyl oleate was 95% for immobilized enzyme. Maximum yield was obtained at 49°C, molar ratio oleic acid:ethanol of 1:3, 1.25 g lipase film or 50 U (1.03 ± 0.03 mg protein) and 30 h. Even after seven cycles of use, immobilized lipase showed 52% reduction in ester yield. Biodegradable and biorenewable film is a promising material as a support to immobilize lipases and application in biocatalysis.

Keywords: adsorption; enzyme-film-enzyme; esterification; factorial design; lipase; starch films.

MeSH terms

  • Biocatalysis
  • Enzyme Stability
  • Enzymes, Immobilized / metabolism
  • Esterification
  • Lipase* / metabolism
  • Oleic Acid
  • Sericins* / metabolism

Substances

  • Enzymes, Immobilized
  • Sericins
  • Oleic Acid
  • Lipase