Native Gel Electrophoresis and Immunoblotting to Analyze Electron Transport Chain Complexes

Gisela Beutner; George A Porter Jr

doi:10.1007/978-1-0716-1266-8_7

Native Gel Electrophoresis and Immunoblotting to Analyze Electron Transport Chain Complexes

Methods Mol Biol. 2021:2276:103-112. doi: 10.1007/978-1-0716-1266-8_7.

Authors

Gisela Beutner¹, George A Porter Jr²

Affiliations

¹ Department of Pediatrics-Division Cardiology, University of Rochester, Rochester, NY, USA.
² Departments of Pediatrics-Division Cardiology, Pharmacology and Physiology, and Medicine (Aab Cardiovascular Research Institute), University of Rochester, Rochester, NY, USA. George_Porter@urmc.rochester.edu.

PMID: 34060035
DOI: 10.1007/978-1-0716-1266-8_7

Abstract

Native electrophoresis is a powerful tool to analyze the mitochondrial electron transport chain complexes (Cx) I-V and their assembly into supercomplexes. Valuable information regarding the composition and bioenergetic regulation in physiological and pathological conditions can be obtained. This chapter compares different types of native electrophoresis to analyze mitochondrial supercomplexes.

Keywords: Mitochondrial supercomplexes; Native electrophoresis (blue native, colorless native, clear native, hybrid).

MeSH terms

Animals
Electron Transport
Electron Transport Chain Complex Proteins / chemistry
Electron Transport Chain Complex Proteins / metabolism*
Electrophoresis, Gel, Two-Dimensional / methods*
Electrophoresis, Polyacrylamide Gel / methods*
Humans
Immunoblotting / methods*
Mitochondria / metabolism*
Mitochondrial Membranes / metabolism*
Mitochondrial Proteins / chemistry
Mitochondrial Proteins / metabolism*

Substances

Electron Transport Chain Complex Proteins
Mitochondrial Proteins