Assessment of Mitochondrial Ca2+ Uptake

András T Deak; Claire Jean-Quartier; Alexander I Bondarenko; Lukas N Groschner; Roland Malli; Wolfgang F Graier; Markus Waldeck-Weiermair

doi:10.1007/978-1-0716-1266-8_13

Assessment of Mitochondrial Ca²⁺ Uptake

Methods Mol Biol. 2021:2276:173-191. doi: 10.1007/978-1-0716-1266-8_13.

Authors

András T Deak¹, Claire Jean-Quartier¹, Alexander I Bondarenko¹, Lukas N Groschner¹, Roland Malli¹, Wolfgang F Graier¹, Markus Waldeck-Weiermair²

Affiliations

¹ Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Molecular Biology and Biochemistry, Medical University of Graz, Graz, Austria.
² Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Molecular Biology and Biochemistry, Medical University of Graz, Graz, Austria. markus.weiermair@medunigraz.at.

PMID: 34060041
DOI: 10.1007/978-1-0716-1266-8_13

Abstract

Mitochondrial Ca²⁺ uptake regulates mitochondrial function and contributes to cell signaling. Accordingly, quantifying mitochondrial Ca²⁺ signals and elaborating the mechanisms that accomplish mitochondrial Ca²⁺ uptake are essential to gain our understanding of cell biology. Here, we describe the benefits and drawbacks of various established old and new techniques to assess dynamic changes of mitochondrial Ca²⁺ concentration ([Ca²⁺]_mito) in a wide range of applications.

Keywords: Ca2+ Imaging; Calcium Green; FRET; Fura-2; Mitochondrial Ca2+ uptake; Mitochondrial membrane potential; Mitoplast; Oxidative phosphorylation; Patch-clamp recording; Rhod-2.

MeSH terms

Animals
Calcium / metabolism*
Cells, Cultured
Fluorescent Dyes / chemistry
Humans
Microscopy, Fluorescence / methods*
Mitochondria / metabolism*
Oxygen Consumption / physiology
Patch-Clamp Techniques / methods*

Substances

Fluorescent Dyes
Calcium