Purpose: To investigate the expression of miR-614 in serum of gastric cancer patients and its effect on invasion and proliferation of gastric cancer cell line HGC-27.
Methods: Thirty patients with gastric cancer from May 2016 to November 2018 comprised the research group, and 30 healthy people undergoing physical examination in the same period comprised the control group. The expression of miR-614 in tissues and miR-614 in HGC-27 cell line was detected by qRT-PCR, miR-614-mimics was transfected into HGC-27, while miR-614-mimics group, blank control group and negative control group were established respectively. Cell invasion was detected by Transwell method, and CCK-8 method was used to detect the effect of miR-614 transfection on the proliferation of HGC-27 cells on the 1st, 2nd, 3rd and 4th day.
Results: The expression of miR-614 in the research group was significantly lower than in the control group (p<0.05). The number of cells passing through the invasion microvessel membrane in miR-614-mimics group was significantly lower than in negative control group and blank control group (p<0.05) 24 h after transfection. On the 3rd and 4th day, the cell proliferation of miR-614-mimics group was significantly lower than in blank group and negative control group (p<0.05).
Conclusion: In conclusion, miR-614 is lowly expressed in gastric cancer patients and inhibits the invasion and proliferation of gastric cancer cell line HGC-27.