Objective: To observe the effect of application at Back-Shu with Front-Mu acupoints on serum uric acid (SUA) and kidney uric acid transport related proteins in hyperuricemia rats, so as to explore the mechanism of Shu-Mu acupoint application on treatment of hyperuricemia.
Methods: SD rats were randomly divided into blank control, model, vaseline application and medication application groups, with 8 rats in each group. The hyperuricemia rat model was established by gavage of potassium oxonate. Rats in the vaseline application group received application of vaseline at bilateral "Ganshu"(BL18) and "Qimen"(LI14), "Pishu"(BL20) and "Zhangmen"(LR13), "Shenshu" (BL23) and "Jingmen"(GB25). Rats in the medication application group received application of traditional Chinese medicine at the same acupoints. The contents of SUA and creatinine (SCr) were detected by automatic biochemical analyzer. H.E. staining was used to observe the pathological changes of kidney. And the protein expression levels of organic anion transporter 1(OAT1) and adenosine triphosphate binding cassette transporter G2(ABCG2) were detected by immunohistochemistry.
Results: Rats in the model group showed symptoms such as polydipsia, polyuria, loose stools, fatigue, weakness, etc. The renal tubules atrophied, and urate crystals can be seen in the lumen. Compared with the control group. the SUA content in the model group increased (P<0.01)and the expressions of OAT1 and ABCG2 protein in kidney decreased (P<0.01). After intervention and in comparison with the model group showed that, the diet, excretion function, and mental state of the rats in the medication application group returned to normal, and the pathological changes of the kidney tissue were alleviated, the SUA content was down-regulated(P<0.01)and the expression levels of OAT1 and ABCG2 in the kidney up-regulated (P<0.01). There was no statistically significant difference in the SCr content among the 4 groups (P＞0.05).
Conclusion: Medication application at Shu-Mu points can effectively reduce the SUA level of hyperuricemia rats, which may be related to its effects in up-regulating the protein expressions of OAT1 and ABCG2 in the kidney and reducing the damage to the kidneys.
目的：通过观察俞募穴贴敷对高尿酸血症大鼠血尿酸和肾脏尿酸转运相关蛋白的影响, 探讨其作用机制。方法：SD大鼠随机分为空白组、模型组、凡士林贴敷组、药物贴敷组, 每组8只。采用氧嗪酸钾灌胃的方式制备高尿酸血症大鼠模型。凡士林贴敷组用凡士林贴敷双侧“肝俞”“期门”“脾俞”“章门”“肾俞”和“京门”, 药物贴敷组采用由柴胡、芍药、熟地黄、泽泻、茯苓、白术、白芥子等方药组成的药物贴贴敷相同的穴位,6 h/次, 1次/d, 5次为1个疗程, 治疗3个疗程。用全自动生化分析仪检测大鼠血清尿酸(SUA)、肌酐(SCr)含量, HE染色法观察大鼠肾组织病理学变化, 免疫组织化学法检测大鼠肾脏有机阴离子转运体1(OAT1)和三磷酸腺苷结合盒转运蛋白G2(ABCG2)的蛋白表达水平。结果：模型组大鼠出现多饮、多尿、大便稀, 倦怠无力, 精神萎靡等症状, 肾小管萎缩, 管腔内可见尿酸盐结晶；与空白组比较, 模型组大鼠SUA含量增高(P<0.01)；肾脏OAT1和ABCG2蛋白阳性表达减少(P<0.01)。与模型组比较, 药物贴敷组大鼠饮食、排泄功能、精神状态等基本恢复正常, 肾脏的病理变化减轻；SUA含量降低(P<0.01)；肾脏OAT1和ABCG2表达水平升高(P<0.01)。各组大鼠SCr含量差异无统计学意义(P＞0.05)。结论：俞募穴药物贴敷可有效降低高尿酸血症大鼠SUA水平, 其机制可能是通过增加大鼠肾组织中OAT1和ABCG2的蛋白表达, 降低对肾脏的损害。.
Keywords: Application; Hyperuricemia; Serum uric acid; Shu-Mu acupoint; Uric acid transport related protein; stroke.