Rapid identification of bacteria from respiratory samples of patients hospitalized in intensive care units, with FilmArray Pneumonia Panel Plus

François Caméléna; Thibaut Poncin; Emmanuel Dudoignon; Maud Salmona; Jérôme Le Goff; Jean-Luc Donay; Matthieu Lafaurie; Michael Darmon; Elie Azoulay; Benoît Plaud; Alexandre Mebazaa; François Dépret; Hervé Jacquier; Béatrice Berçot

doi:10.1016/j.ijid.2021.05.074

Rapid identification of bacteria from respiratory samples of patients hospitalized in intensive care units, with FilmArray Pneumonia Panel Plus

Int J Infect Dis. 2021 Jul:108:568-573. doi: 10.1016/j.ijid.2021.05.074. Epub 2021 Jun 1.

Affiliations

¹ Department of Bacteriology, Saint-Louis-Lariboisière Hospital Group, Assistance Publique-Hôpitaux de Paris, Paris, France; University of Paris, INSERM 1137, IAME, Paris, France.
² Department of Anaesthesiology and Critical Care and Burns Unit, Saint-Louis-Lariboisière Hospital Group, Assistance Publique - Hôpitaux de Paris, Paris, France; University of Paris, FHU PROMICE, INSERM 942, INI-CRCT Network, Paris, France.
³ University of Paris, Inserm U976, Insight team, F-75010, Paris France; Department of Virology, Saint-Louis-Lariboisière Hospital Group, Assistance Publique-Hôpitaux de Paris, Paris, France.
⁴ Department of Bacteriology, Saint-Louis-Lariboisière Hospital Group, Assistance Publique-Hôpitaux de Paris, Paris, France.
⁵ Department of Infectious Disease, Saint-Louis-Lariboisière Hospital Group, Assistance Publique - Hôpitaux de Paris, Paris, France.
⁶ Medical Intensive Care Unit, Saint-Louis-Lariboisière Hospital Group, Assistance Publique - Hôpitaux de Paris, Paris, France; University of Paris, INSERM 1153, Centre of Epidemiology and Biostatistics, ECSTRA Team, Paris, France.
⁷ Department of Bacteriology, Saint-Louis-Lariboisière Hospital Group, Assistance Publique-Hôpitaux de Paris, Paris, France; University of Paris, INSERM 1137, IAME, Paris, France. Electronic address: beatrice.bercot@aphp.fr.

PMID: 34087488
DOI: 10.1016/j.ijid.2021.05.074

Abstract

Objectives: This study aimed to evaluate the performance of FilmArray Pneumonia Panel Plus (FA-PP) for the detection of typical bacterial pathogens in respiratory samples from patients hospitalized in intensive care units (ICUs).

Methods: FA-PP was implemented for clinical use in the microbiology laboratory in March 2020. A retrospective analysis on a consecutive cohort of adult patients hospitalized in ICUs between March 2020 and May 2020 was undertaken. The respiratory samples included sputum, blind bronchoalveolar lavage (BBAL) and protected specimen brush (PSB). Conventional culture and FA-PP were performed in parallel.

Results: In total, 147 samples from 92 patients were analysed; 88% had coronavirus disease 2019 (COVID-19). At least one pathogen was detected in 46% (68/147) of samples by FA-PP and 39% (57/147) of samples by culture. The overall percentage agreement between FA-PP and culture results was 98% (93-100%). Bacteria with semi-quantitative FA-PP results ≥10⁵ copies/mL for PSB samples, ≥10⁶ copies/mL for BBAL samples and ≥10⁷ copies/mL for sputum samples reached clinically significant thresholds for growth in 90%, 100% and 91% of cultures, respectively. FA-PP detected resistance markers, including mecA/C, bla_CTX-M and bla_VIM. The median turnaround time was significantly shorter for FA-PP than for culture.

Conclusions: FA-PP may constitute a faster approach to the diagnosis of bacterial pneumonia in patients hospitalized in ICUs.

Keywords: Bacterial pneumonia; Blind bronchoalveolar lavage; Community-acquired pneumonia; FilmArray Pneumonia plus; Hospital-acquired pneumonia; Rapid diagnostics; SARS-CoV-2.

MeSH terms

Adult
Bacteria
COVID-19*
Humans
Intensive Care Units
Pneumonia*
Pneumonia, Bacterial* / diagnosis
Retrospective Studies
SARS-CoV-2