Regulated expression of a gene encoding a nuclear factor, IRF-1, that specifically binds to IFN-beta gene regulatory elements

Cell. 1988 Sep 9;54(6):903-13. doi: 10.1016/s0092-8674(88)91307-4.


Expression of the interferon-beta (IFN-beta) gene is induced by a variety of agents, including viruses. Evidence has been provided that a mouse nuclear factor, termed interferon regulatory factor-1 (IRF-1), specifically binds to the upstream regulatory region of the human IFN-beta gene and mediates virus-induced transcription of the gene. In this study, we describe the molecular cloning and characterization of the mouse and human cDNAs encoding IRF-1. Our results suggest that IRF-1 is also involved in the regulation of other genes such as IFN-alpha and MHC class I genes. Surprisingly, IRF-1 gene expression is dramatically induced by Newcastle disease virus in mouse L929 cells and by Concanavalin A in spleen cells. We show here that the IRF-1 gene possesses virus-inducible promoter.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Cloning, Molecular
  • Concanavalin A / pharmacology
  • DNA / genetics
  • DNA-Binding Proteins / genetics*
  • Gene Expression Regulation* / drug effects
  • Humans
  • Interferon Type I / genetics*
  • Mice
  • Molecular Sequence Data
  • Newcastle disease virus
  • Promoter Regions, Genetic*
  • RNA, Messenger / genetics
  • Regulatory Sequences, Nucleic Acid*
  • Transcription Factors / genetics*


  • DNA-Binding Proteins
  • Interferon Type I
  • RNA, Messenger
  • Transcription Factors
  • Concanavalin A
  • DNA

Associated data

  • GENBANK/J03160
  • GENBANK/M21065
  • GENBANK/M21066