Analytical comparison of ELISA and mass spectrometry for quantification of serum hepcidin in critically ill patients

Bioanalysis. 2021 Jul;13(13):1029-1035. doi: 10.4155/bio-2021-0069. Epub 2021 Jun 8.


Aim: To compare methods of quantifying serum hepcidin (based on MS and ELISA) and their ability to diagnose true iron deficiency anemia in critically ill patients. Materials & methods: Serum hepcidin was measured in 119 critically ill patients included in the HEPCIDANE clinical trial, using either an ultra-sensitive ELISA kit (from DRG) or two different MS methods. Results: The results show a good correlation between the different methods studied. The Bland-Altman analysis and the Kappa test for clinical groups show a good or very good agreement between the different tests. Conclusion: ELISA or MS show a satisfactory commutability to quantify serum hepcidin. This is of great importance for the determination of therapeutic strategies in iron deficiency.

Keywords: ELISA; anemia; hepcidin; mass spectrometry.

Publication types

  • Comparative Study

MeSH terms

  • Anemia, Iron-Deficiency / blood
  • Anemia, Iron-Deficiency / diagnosis
  • Anemia, Iron-Deficiency / etiology*
  • Critical Illness
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Hepcidins / blood*
  • Humans
  • Mass Spectrometry / methods*
  • Protein Isoforms / blood
  • Reagent Kits, Diagnostic


  • Hepcidins
  • Protein Isoforms
  • Reagent Kits, Diagnostic