Production of a specific antibody against pyruvate kinase type M2 using a synthetic peptide

FEBS Lett. 1988 Aug 29;236(2):391-5. doi: 10.1016/0014-5793(88)80063-2.

Abstract

The pyruvate kinase isozymes M1 and M2 are structurally and immunologically closely related. To obtain an antibody which discriminates between these two forms, a synthetic tetradecapeptide with a sequence specific for pyruvate kinase type M2 from rats was constructed. Antisera from rabbits, immunized with this peptide, reacted specifically with the M2-type holoenzyme of both rat and human origin, and did not cross-react with the M1-type isozyme. This was established by immunoblot analysis, both under dissociating and non-dissociating conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies / immunology*
  • Antibody Specificity
  • Isoenzymes / immunology
  • Molecular Sequence Data
  • Oligopeptides / chemical synthesis
  • Oligopeptides / immunology
  • Pyruvate Kinase / immunology*
  • Rats

Substances

  • Antibodies
  • Isoenzymes
  • Oligopeptides
  • Pyruvate Kinase