Role of qGZn9a in controlling grain zinc concentration in rice, Oryza sativa L

Theor Appl Genet. 2021 Sep;134(9):3013-3022. doi: 10.1007/s00122-021-03873-4. Epub 2021 Jun 10.

Abstract

A candidate gene responsible for higher grain zinc accumulation in rice was identified, which was probably associated with a partial defect in anther dehiscence. Zinc (Zn) is an essential mineral element in many organisms. Zn deficiency in humans causes various health problems; therefore, an adequate dietary Zn intake is required daily. Rice, Oryza sativa, is one of the main crops cultivated in Asian countries, and one of the breeding scopes of rice is to increase the grain Zn levels. Previously, we found that an Australian wild rice strain, O. meridionalis W1627, exhibits higher grain Zn levels than cultivated rice, O. sativa Nipponbare, and identified responsible genomic loci. An increase in grain Zn levels caused by one of the loci, qGZn9a, is associated with fertility reduction, but how this negative effect on grain productivity is regulated remains unknown. In this study, we artificially trimmed spikelets on the flowering day and found that a reduction in number of seeds was associated with an increase in the grain Zn levels. We also found that a partial defect in anther dehiscence correlated with the increase in grain Zn levels in plants carrying the W1627 chromosomal segment at qGZn9a in a Nipponbare genetic background. Among eight candidate genes in the qGZn9a region, three were absent from the corresponding region of W1627; one of these, Os09g0384900, encoding a DUF295 protein with an unknown function, was found to be specifically expressed in the developing anther, thereby suggesting that the gene may be involved in the regulation of anther dehiscence. As fertility and grain Zn levels are essential agronomic traits in rice, our results highlight the importance of balancing these two traits.

Keywords: Anther dehiscence; Fertility; Grain zinc concentration; Oryza sativa.

MeSH terms

  • Australia
  • Chromosome Mapping / methods*
  • Chromosomes, Plant / genetics*
  • Edible Grain / genetics
  • Edible Grain / growth & development
  • Edible Grain / metabolism*
  • Gene Expression Regulation, Plant
  • Oryza / genetics
  • Oryza / growth & development
  • Oryza / metabolism*
  • Phenotype
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Quantitative Trait Loci*
  • Zinc / analysis
  • Zinc / metabolism*

Substances

  • Plant Proteins
  • Zinc