A novel SERS sensor for the ultrasensitive detection of kanamycin based on a Zn-doped carbon quantum dot catalytic switch controlled by nucleic acid aptamer and size-controlled gold nanorods

Ximo Wang; Chen Chen; Geoffrey I N Waterhouse; Xuguang Qiao; Zhixiang Xu

doi:10.1016/j.foodchem.2021.130261

A novel SERS sensor for the ultrasensitive detection of kanamycin based on a Zn-doped carbon quantum dot catalytic switch controlled by nucleic acid aptamer and size-controlled gold nanorods

Food Chem. 2021 Nov 15:362:130261. doi: 10.1016/j.foodchem.2021.130261. Epub 2021 Jun 1.

Authors

Ximo Wang¹, Chen Chen¹, Geoffrey I N Waterhouse², Xuguang Qiao¹, Zhixiang Xu³

Affiliations

¹ Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China.
² School of Chemical Sciences, The University of Auckland, Auckland 1142, New Zealand.
³ Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China. Electronic address: zhixiangxu@sina.com.

PMID: 34111691
DOI: 10.1016/j.foodchem.2021.130261

Abstract

In this study, a novel surface enhanced Raman spectroscopy (SERS) sensor was developed for the ultrasensitive determination of kanamycin in foods. The sensor used two distinct signal amplification strategies, namely the surface plasmon resonance of gold nanorods and a Zn-doped carbon quantum dots catalytic cascade oxidation-reduction reaction switch controlled by a nucleic acid aptamer. Under optimized experimental conditions, the SERS sensor demonstrated a linear range of 10^-12 to 10^-5 g mL^-1 for the detection of kanamycin, with a limit of detection of 3.03 × 10^-13 g mL^-1. Experiments with antibiotics structurally similar to kanamycin and interferrants revealed that the sensor had excellent selectivity. Milkpowder and honey samples spiked with kanamycin were assayed, with recoveries ranging from 84.1% to 107.2% and a relative standard deviation of 0.74% to 2.81% being obtained. Quantification of kanamycin in milk samples revealed no significant difference between the results obtained with the sensor and by HPLC.

Keywords: Catalytic switch; Kanamycin; Metal-doped carbon quantum dots; Plasmon resonance; Raman substrate.

MeSH terms

Anti-Bacterial Agents / analysis
Aptamers, Nucleotide / chemistry*
Biosensing Techniques / instrumentation
Carbon / chemistry
Catalysis
Food Analysis / instrumentation*
Food Analysis / methods*
Gold / chemistry
Kanamycin / analysis*
Limit of Detection
Metal Nanoparticles / chemistry
Nanotubes / chemistry*
Quantum Dots / chemistry*
Spectrum Analysis, Raman / methods
Surface Plasmon Resonance
Zinc / chemistry*

Substances

Anti-Bacterial Agents
Aptamers, Nucleotide
Kanamycin
Carbon
Gold
Zinc