Protein Arrays for the Identification of Seroreactive Protein Markers for Infectious Diseases

Methods Mol Biol. 2021:2344:139-150. doi: 10.1007/978-1-0716-1562-1_10.


The protein array is a powerful platform to study humoral responses to infectious agents using small sample volumes [<3 μL]. Its success can be largely attributed to the development of new strategies for high-throughput cloning and expression, and improved manufacturing techniques for the construction of arrays. Here, we describe a method to hybridize protein arrays with malaria patients' sera in order to identify seroreactive antigens, some of which may have a high potential of conferring protection from severe forms of malaria.

Keywords: Malaria exposure; Plasmodium falciparum; Plasmodium vivax; Protective immunity; Protein arrays; Seroreactive antigens.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / analysis
  • Humans
  • Malaria / diagnosis*
  • Malaria / immunology
  • Protein Array Analysis*
  • Protozoan Proteins / analysis*
  • Protozoan Proteins / immunology
  • Serologic Tests*


  • Biomarkers
  • Protozoan Proteins