Isolation, structural characterization and anti-oxidant activity of a novel polysaccharide from garlic bolt

Guoqiang Li; Peifang Chen; Yiting Zhao; Qiaohui Zeng; Shiyi Ou; Yinghui Zhang; Pengcheng Wang; Nenghua Chen; Juanying Ou

doi:10.1016/j.carbpol.2021.118194

Isolation, structural characterization and anti-oxidant activity of a novel polysaccharide from garlic bolt

Carbohydr Polym. 2021 Sep 1:267:118194. doi: 10.1016/j.carbpol.2021.118194. Epub 2021 May 13.

Authors

Guoqiang Li¹, Peifang Chen², Yiting Zhao², Qiaohui Zeng², Shiyi Ou³, Yinghui Zhang², Pengcheng Wang⁴, Nenghua Chen⁵, Juanying Ou⁶

Affiliations

¹ Department of Food Science, Foshan University, Foshan 528000, Guangdong, PR China; South China National Center for Food Safety Research and Development, Foshan 528000, Guangdong, PR China.
² Department of Food Science, Foshan University, Foshan 528000, Guangdong, PR China.
³ Department of Food Science and Engineering, Jinan University, Guangzhou 510632, Guangdong, PR China.
⁴ Foshan Nuorun Biotechnology Co. LTD, Foshan 528000, Guangdong, PR China.
⁵ Institute of Traditional Chinese Medicine & Natural Products, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 511443, Guangdong, PR China. Electronic address: chennengflo@163.com.
⁶ Department of Food Science and Engineering, Jinan University, Guangzhou 510632, Guangdong, PR China. Electronic address: toujy@jnu.edu.cn.

PMID: 34119161
DOI: 10.1016/j.carbpol.2021.118194

Abstract

Allium sativum L. is a widely distributed plant used as a spice, vegetable and medicine. In this study, one novel water-soluble polysaccharide (GBP-1a), with a molecular weight of 15.0 kDa, was isolated from the scape of A. sativum (garlic bolt). GBP-1a consists of galactose, glucose and arabinose at a ratio of 73.29:4.36:1.70. It has a backbone, which is composed of 1,4-linked Galp, with 1,2,6-linked Galp branches and 1-linked Glcp residue. In addition, the anti-oxidant activities of GBP-1a, as well as the two main polysaccharide fractions on ABTS radicals, metal ions and superoxide anion radicals, were evaluated in vitro. This study added new data to the study of polysaccharides from garlic bolt.

Keywords: Anti-oxidant activity; Garlic bolt; Polysaccharides; Structural characterization.

MeSH terms

Carbohydrate Sequence
Free Radical Scavengers / chemistry*
Free Radical Scavengers / isolation & purification
Garlic / chemistry*
Molecular Weight
Polysaccharides / chemistry*
Polysaccharides / isolation & purification

Substances

Free Radical Scavengers
Polysaccharides