A Fast PCR Test for the Simultaneous Identification of Species and Gender in Horses, Donkeys, Mules and Hinnies

Xisheng Wang; Gerelchimeg Bou; Xinzhuang Zhang; Li Tao; Yingchao Shen; Riga Na; Guiqin Liu; Hong Ren; Xiujuan Ren; Lianjie Song; Shaofeng Su; Dongyi Bai; Yiping Zhao; Bei Li; Manglai Dugarjaviin

doi:10.1016/j.jevs.2021.103458

A Fast PCR Test for the Simultaneous Identification of Species and Gender in Horses, Donkeys, Mules and Hinnies

J Equine Vet Sci. 2021 Jul:102:103458. doi: 10.1016/j.jevs.2021.103458. Epub 2021 Apr 27.

Authors

Affiliations

¹ College of animal science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, China.
² College of animal science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, China; College of Agronomy, Liaocheng University, Shandong Engineering Technology Research Center for Efficient Breeding and Ecological Feeding of Black Donkey, Shandong Donkey Industry Technology Collaborative Innovation Center, Liaocheng, Shandong Province, China.
³ College of animal science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, China; Vocational and Technical College, Inner Mongolia Agricultural University, Hohhot, China.
⁴ College of animal science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, China. Electronic address: dmanglai@163.com.

PMID: 34119210
DOI: 10.1016/j.jevs.2021.103458

Abstract

Having considered that the current methods are costly and time-consuming, we designed an only 3 pairs primer-based PCR test to accurately identify the species and gender in horses, donkeys, mules and hinnies. Through a thorough sequence comparison between horse and donkey's highly similar genomes, and a vast amount of preliminary confirmation, we found that three fragments, CNGB3 gene on an autosome, displacement loop region on mitochondrial DNA and SRY genes on chromosome Y, within these equine DNA, are enough to enable us achieving our goal. The PCR test described here would be an economical, fast and accurate alternative for the most commonly-used methods, polymerase chain reaction-restriction fragment length polymorphism, microsatellite assay, and sequencing.

Keywords: Donkey; Hinny; Horse; Identification; Mule.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Equidae* / genetics
Horses / genetics
Microsatellite Repeats / genetics
Polymerase Chain Reaction / veterinary
Polymorphism, Restriction Fragment Length
Y Chromosome*