A near-infrared fluorescence probe for imaging of pantetheinase in cells and mice in vivo

Yuantao Yang; Yiming Hu; Wen Shi; Huimin Ma

doi:10.1039/d0sc04537c

A near-infrared fluorescence probe for imaging of pantetheinase in cells and mice in vivo

Chem Sci. 2020 Sep 25;11(47):12802-12806. doi: 10.1039/d0sc04537c.

Authors

Yuantao Yang^{1

2}, Yiming Hu¹, Wen Shi^{1

2}, Huimin Ma^{1

2}

Affiliations

¹ Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences Beijing 100190 China shiwen@iccas.ac.cn.
² University of Chinese Academy of Sciences Beijing 100049 China.

Abstract

Pantetheinase is an amidohydrolase that cleaves pantetheine into pantothenic acid and cysteamine. Functional studies have found that ubiquitous expression of this enzyme is associated with many inflammatory diseases. However, the lack of near-infrared fluorescence probes limits the better understanding of the functions of the enzyme. In this work, we have developed a new near-infrared fluorescence probe, CYLP, for bioimaging of pantetheinase by using pantothenic acid with a self-immolative linker as a recognition group. The probe produces a sensitive fluorescence off-on response at 710 nm to pantetheinase with a detection limit of 0.02 ng mL^-1 and can be used to image the intraperitoneal pantetheinase activity in mice in vivo. Moreover, with the probe we have observed that pantetheinase is significantly increased in the tissues of mouse inflammatory models as well as in the intestines of mice with inflammatory bowel disease. Therefore, CYLP may provide a convenient and intuitive tool for studying the role of pantetheinase in diseases.