The alpha- and beta-tubulins of Toxoplasma gondii are encoded by single copy genes containing multiple introns

Mol Biochem Parasitol. 1988 Jun;29(2-3):261-73. doi: 10.1016/0166-6851(88)90081-3.

Abstract

We have studied the fundamentals of gene expression in the protozoan parasite Toxoplasma gondii by analyzing, in detail, the genes encoding alpha- and beta-tubulin. Southern analysis and quantitation studies reveal that, unlike in other organisms studied thus far, both these genes are present as single copies in the haploid Toxoplasma genome. Sequencing of these genes indicates that they both contain multiple introns with conserved 5' and 3' splice site signals. We have found that HeLa cell nuclear extracts are able to accurately splice a Toxoplasma pre-mRNA construct. We have mapped, for the alpha-tubulin gene, the exact site of transcription initiation and the approximate site of poly A addition by primer extension and RNase protection assays. Trans-splicing, as demonstrated in the Kinetoplastida, is not involved in the formation of the mature alpha-tubulin transcript in T. gondii.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA / genetics
  • Gene Expression Regulation*
  • HeLa Cells
  • Humans
  • Introns*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • RNA, Messenger / genetics
  • Sequence Homology, Nucleic Acid
  • Toxoplasma / genetics*
  • Transcription, Genetic
  • Tubulin / genetics*

Substances

  • RNA, Messenger
  • Tubulin
  • DNA

Associated data

  • GENBANK/M20024
  • GENBANK/M20025