Platycodin D attenuates airway inflammation via suppression Th2 transcription factor in a murine model of acute asthma

J Asthma. 2022 Jul;59(7):1279-1289. doi: 10.1080/02770903.2021.1941084. Epub 2021 Jun 23.

Abstract

Introduction: Bronchial asthma is a common chronic inflammatory condition of the airway tissue. Platycodin D (PLD) has antiinflammatory effects in a mouse model of allergic asthma. In this work, the anti-asthma potential of PLD was studied by investigation of its effect to suppress airway inflammation and mucin production, a murine model of asthma and the possible mechanisms.Methods: Mice were randomly assigned to five experimental groups: control, ovalbumin (OVA), OVA+ICS (intranasal fluticasone), OVA+PLD and OVA+PLD/ICS. Airway histological studies were evaluated by the H&E staining; IL-4, IL-5, and IL-13 in bronchoalveolar lavage fluid were evaluated by ELISA; GATA3 and IRF4 mRNA of airway were measured by RT-PCR and their protein level were measured by Western blotting.Results: Our study showed that PLD suppressed eosinophilic inflammation and mucin production in bronchial mucosa. Moreover, PLD inhibited production of Th2 cytokines such as IL-4, IL-5, and IL-13. Protein production of GATA3 and IRF4, were also decreased in PLD treated OVA asthma model. Taken together, our results provided evidence that PLD inhibits the airway inflammation via suppression of Th2 transcription factor production.Conclusion: These findings suggest that PLD may effectively ameliorate the progression of asthma. These results suggest that PLD could be used as a therapy for allergic asthma.

Keywords: Platycodon grandiflorum; asthma; inflammation; platycodin D.

MeSH terms

  • Animals
  • Asthma* / pathology
  • Bronchoalveolar Lavage Fluid
  • Cytokines / metabolism
  • Disease Models, Animal
  • Inflammation / drug therapy
  • Inflammation / pathology
  • Interleukin-13
  • Interleukin-4 / metabolism
  • Interleukin-5 / metabolism
  • Lung / pathology
  • Mice
  • Mucins / metabolism
  • Mucins / pharmacology
  • Ovalbumin / pharmacology
  • Saponins
  • Status Asthmaticus*
  • Transcription Factors / metabolism
  • Transcription Factors / pharmacology
  • Triterpenes

Substances

  • Cytokines
  • Interleukin-13
  • Interleukin-5
  • Mucins
  • Saponins
  • Transcription Factors
  • Triterpenes
  • Interleukin-4
  • Ovalbumin
  • platycodin D