Transcriptome analysis of Sonneratia caseolaris seedlings under chilling stress

PeerJ. 2021 Jun 3:9:e11506. doi: 10.7717/peerj.11506. eCollection 2021.

Abstract

Sonneratia caseolaris is a native mangrove species found in China. It is fast growing and highly adaptable for mangrove afforestation, but suffered great damage by chilling event once introduced to high latitude area. To understand the response mechanisms under chilling stress, physiological and transcriptomic analyses were conducted. The relative electrolyte conductivity, malondialdehyde (MDA) content, soluble sugar content and soluble protein content increased significantly under chilling stress. This indicated that S. caseolaris suffered great damage and increased the levels of osmoprotectants in response to the chilling stress. Gene expression comparison analysis of S. caseolaris leaves after 6 h of chilling stress was performed at the transcriptional scale using RNA-Seq. A total of 168,473 unigenes and 3,706 differentially expressed genes (DEGs) were identified. GO and KEGG enrichment analyses showed that the DEGs were mainly involved in carbohydrate metabolism, antioxidant enzyme, plant hormone signal transduction, and transcription factors (TFs). Sixteen genes associated with carbohydrate metabolism, antioxidant enzyme, phytohormones and TFs were selected for qRT-PCR verification, and they indicated that the transcriptome data were reliable. Our work provided a comprehensive review of the chilling response of S. caseolaris at both physiological and transcriptomic levels, which will prove useful for further studies on stress-responses in mangrove plants.

Keywords: Chilling stress; Differentially expressed genes; RNA-Seq; Sonneratia caseolaris.

Grants and funding

This study was supported by grants from Hainan Natural Science Foundation (Grant No.319QN213) and the National Natural Science Foundation of China (Grant No. 31960281, 32071503). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.