Characterization of the biosurfactant produced by Pesudomonas areuginosa strain R 4 and its application for remediation pyrene-contaminated soils

Mehdi Ahmadi; Forud Niazi; Neematollah Jaafarzadeh; Shokouh Ghafari; Sahand Jorfi

doi:10.1007/s40201-021-00617-w

Characterization of the biosurfactant produced by Pesudomonas areuginosa strain R ₄ and its application for remediation pyrene-contaminated soils

J Environ Health Sci Eng. 2021 Jan 27;19(1):445-456. doi: 10.1007/s40201-021-00617-w. eCollection 2021 Jun.

Authors

Mehdi Ahmadi^{1

2}, Forud Niazi², Neematollah Jaafarzadeh^{1

2}, Shokouh Ghafari³, Sahand Jorfi^{1

2}

Affiliations

¹ Environmental Technologies Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
² Department of Environmental Health Engineering, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
³ Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.

Abstract

A biosurfactant producing strain was isolated and the rhamnolipid type biosurfactant was extracted for soil washing of a synthetically and naturally hydrocarbon-contaminated soil. Following the primary screening, Pseudomonas aeruginosa strain R ₄ was selected and the effect of the carbon and nitrogen source and the salinity on biosurfactant production was studied. Of the best results were observed for glucose as a carbon source, NH₄Cl as a nitrogen source and salinity of 1.4%. The produced biosurfactant was a glycolipid type biosurfactant and reduced the surface tension to 32.5 mN/m with a critical micelle concentration (CMC) of 50 mg/L and production yield of 90 mg/L. Using produced biosurfactant, a pyrene desorption rate of 82% was observed in selected conditions for initial pyrene concentration of 200 mg/L.

Keywords: Biosurfactant; Pseudomonas aeruginosa strain R4; Pyrene; Soil pollution; Soil washing.