Techniques of orthotopic renal transplantation. II. Size-matched porcine grafts in monkey recipients

Acta Cir Bras. 2021 Jun 18;36(5):e360503. doi: 10.1590/ACB360503. eCollection 2021.


Purpose: As a classical xenotransplantation model, porcine kidneys have been transplanted into the lower abdomen of non-human primates. However, we have improved upon this model by using size-matched grafting in the orthotopic position. The beneficial aspects and surgical details of our method are reported herein.

Methods: Donors were two newborn pigs (weighting 5 to 6 kg) and recipients were two cynomolgus monkeys (weighting, approximately, 7 kg). After bilateral nephrectomy, kidneys were cold-transported in Euro-Collins solution. The porcine kidney was transplanted to the site of a left nephrectomy and fixed to the peritoneum.

Results: Kidneys transplanted to the lower abdomen by the conventional method were more susceptible to torsion of the renal vein (two cases). In contrast, early-stage blood flow insufficiency did not occur in orthotopic transplants of theleft kidney.

Conclusions: Size-matched porcine-primate renal grafting using our method of transplanting tothe natural position of the kidneys contributes to stable post-transplant blood flow to the kidney.

MeSH terms

  • Animals
  • Graft Survival
  • Kidney / surgery
  • Kidney Transplantation*
  • Macaca fascicularis
  • Nephrectomy
  • Swine
  • Transplants*