Phylogenomic analysis supports the reclassification of Burkholderia novacaledonica as Caballeronia novacaledonica comb. nov

Int J Syst Evol Microbiol. 2021 Jun;71(6). doi: 10.1099/ijsem.0.004843.

Abstract

Burkholderia novacaledonica is a Betaproteobacterial species isolated from ultramafic soils in New Caledonia. The characterization and classification of this species into the Burkholderia genus was done simultaneously with the proposal of the new genus Caballeronia, initially composed of closely related Burkholderia glathei-like species. Thereafter, some reports based on the use of phylogenetic marker genes suggested that B. novacaledonica forms part of Caballeronia genus. Lacking a formal validation, and with the availability of its genome sequence, a genome-based phylogeny of B. novacaledonica was obtained to unravel its taxonomic position in Burkholderia sensu lato. A partial gyrB gene phylogeny, extended multilocus sequence typing on homologous protein sequences, and genomic distance-based phylogeny, all support the placement of this species in the Caballeronia genus. Therefore, the reclassification of B. novacaledonica to Caballeronia novacaledonica comb. nov. is proposed.

Keywords: Burkholderia; Caballeronia novacaledonica; genome; phylogeny.

MeSH terms

  • Bacterial Typing Techniques
  • Base Sequence
  • Burkholderia / classification*
  • Burkholderia / genetics*
  • Burkholderiaceae / classification
  • Burkholderiaceae / genetics
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Fatty Acids / chemistry
  • Multilocus Sequence Typing
  • New Caledonia
  • Phylogeny*
  • Sequence Analysis, DNA
  • Soil Microbiology

Substances

  • DNA, Bacterial
  • Fatty Acids

Supplementary concepts

  • Burkholderia novacaledonica
  • Caballeronia glathei