Protocol for the generation of murine bronchiolospheres

Ana Ivonne Vazquez-Armendariz; Werner Seeger; Susanne Herold; Elie El Agha

doi:10.1016/j.xpro.2021.100594

Protocol for the generation of murine bronchiolospheres

STAR Protoc. 2021 Jun 11;2(2):100594. doi: 10.1016/j.xpro.2021.100594. eCollection 2021 Jun 18.

Authors

Ana Ivonne Vazquez-Armendariz¹, Werner Seeger¹, Susanne Herold¹, Elie El Agha¹

Affiliation

¹ Department of Internal Medicine, Universities of Giessen and Marburg Lung Center (UGMLC), Cardio-Pulmonary Institute (CPI), Institute for Lung Health (ILH), member of the German Center for Lung Research (DZL), Justus-Liebig University Giessen, 35392 Giessen, Hessen, Germany.

Abstract

Organoid models have been shown to be valuable tools for studying epithelial-mesenchymal crosstalk during biological and pathological settings. Our data identified ACTA2+ PDGFRα+ repair-supportive mesenchymal cells as an important component of the conducting airway niche. Here, we provide a detailed protocol for culturing airway organoids, or bronchiolospheres, which provide an assessment of the ability of mesenchymal cells to support club-cell growth. For complete details on the use and execution of this protocol, please refer to Moiseenko et al. (2020).

Keywords: Cell Biology; Cell culture; Cell isolation; Flow cytometry/mass cytometry; Organoids; Stem cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bronchioles / cytology*
Coculture Techniques
Epithelial-Mesenchymal Transition
Mice
Naphthalenes / toxicity
Organoids / cytology
Tamoxifen / administration & dosage

Substances

Naphthalenes
Tamoxifen
naphthalene