NADPH is important for isobutanol tolerance in a minimal medium of Saccharomyces cerevisiae

Biosci Biotechnol Biochem. 2021 Aug 25;85(9):2084-2088. doi: 10.1093/bbb/zbab115.

Abstract

We showed that the isobutanol sensitivity in glucose-6-phosphate dehydrogenase-deficient cells of the yeast Saccharomyces cerevisiae was rescued by an alternative NADPH producer, acetaldehyde dehydrogenase, but not in the cells lacking 6-phosphogluconate dehydrogenase. This phenotype correlated with the intracellular NADPH/NADP+ ratio in yeast strains. Our findings indicate the importance of NADPH for the isobutanol tolerance of yeast cells.

Keywords: Saccharomyces cerevisiae; 6-phosphogluconate dehydrogenase; NADPH; isobutanol tolerance; pentose phosphate pathway.

MeSH terms

  • Acetaldehyde / metabolism
  • Aldehyde Oxidoreductases / metabolism
  • Butanols / metabolism*
  • Culture Media
  • Fermentation
  • Glucose-6-Phosphate / metabolism
  • NADP / metabolism*
  • Phosphogluconate Dehydrogenase / metabolism
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / metabolism*

Substances

  • Butanols
  • Culture Media
  • NADP
  • Glucose-6-Phosphate
  • isobutyl alcohol
  • Phosphogluconate Dehydrogenase
  • Aldehyde Oxidoreductases
  • aldehyde dehydrogenase (NAD(P)+)
  • Acetaldehyde