Time-resolved X-ray scattering studies of proteins

Curr Opin Struct Biol. 2021 Oct:70:99-107. doi: 10.1016/j.sbi.2021.05.002. Epub 2021 Jun 25.


Time-resolved small- and wide-angle X-ray scattering studies of proteins in solution based on the pump-probe approach unveil structural information from intermediates over a broad range of length and time scales. In spite of the promise of this methodology, only a fraction of the wealth of information encoded in scattering data has been extracted in studies performed thus far. Here, we discuss the methodology, summarize results from recent time-resolved X-ray scattering studies, and examine the potential to extract additional information from these scattering curves.

Keywords: Protein structure dynamics; SAXS; Time-resolved; WAXS.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Proteins*
  • Scattering, Small Angle
  • X-Ray Diffraction
  • X-Rays


  • Proteins