Rhizospheric Bacillus spp. Rescues Plant Growth Under Salinity Stress via Regulating Gene Expression, Endogenous Hormones, and Antioxidant System of Oryza sativa L

Muhammad Aaqil Khan; Muhammad Hamayun; Sajjad Asaf; Murtaza Khan; Byung-Wook Yun; Sang-Mo Kang; In-Jung Lee

doi:10.3389/fpls.2021.665590

Rhizospheric Bacillus spp. Rescues Plant Growth Under Salinity Stress via Regulating Gene Expression, Endogenous Hormones, and Antioxidant System of Oryza sativa L

Front Plant Sci. 2021 Jun 11:12:665590. doi: 10.3389/fpls.2021.665590. eCollection 2021.

Authors

Muhammad Aaqil Khan¹, Muhammad Hamayun², Sajjad Asaf³, Murtaza Khan¹, Byung-Wook Yun², Sang-Mo Kang¹, In-Jung Lee¹

Affiliations

¹ Department of Applied Biosciences, Kyungpook National University, Daegu, South Korea.
² Department of Botany, Abdul Wali Khan University, Mardan, Pakistan.
³ Natural and Medical Science Research Center, University of Nizwa, Nizwa, Oman.

Abstract

Salinity has drastically reduced crop yields and harmed the global agricultural industry. We isolated 55 bacterial strains from plants inhabiting the coastal sand dunes of Pohang, Korea. A screening bioassay showed that 14 of the bacterial isolates secreted indole-3-acetic acid (IAA), 12 isolates were capable of exopolysaccharide (EPS) production and phosphate solubilization, and 10 isolates secreted siderophores. Based on our preliminary screening, 11 bacterial isolates were tested for salinity tolerance on Luria-Bertani (LB) media supplemented with 0, 50, 100, and 150 mM of NaCl. Three bacterial isolates, ALT11, ALT12, and ALT30, had the best tolerance against elevated NaCl levels and were selected for further study. Inoculation of the selected bacterial isolates significantly enhanced rice growth attributes, viz., shoot length (22.8-42.2%), root length (28.18-59%), fresh biomass (44.7-66.41%), dry biomass (85-90%), chlorophyll content (18.30-36.15%), Chl a (29.02-60.87%), Chl b (30.86-64.51%), and carotenoid content (26.86-70%), under elevated salt stress of 70 and 140 mM. Furthermore, a decrease in the endogenous abscisic acid (ABA) content (27.9-23%) and endogenous salicylic acid (SA) levels (11.70-69.19%) was observed in inoculated plants. Antioxidant analysis revealed an increase in total protein (TP) levels (42.57-68.26%), whereas it revealed a decrease in polyphenol peroxidase (PPO) (24.63-34.57%), glutathione (GSH) (25.53-24.91%), SOA (13.88-18.67%), and LPO levels (15.96-26.06%) of bacterial-inoculated plants. Moreover, an increase in catalase (CAT) (26-33.04%), peroxidase (POD) (59.55-78%), superoxide dismutase (SOD) (13.58-27.77%), and ascorbic peroxidase (APX) (5.76-22.74%) activity was observed. Additionally, inductively coupled plasma mass spectrometry (ICP-MS) analysis showed a decline in Na⁺ content (24.11 and 30.60%) and an increase in K⁺ (23.14 and 15.45%) and Mg⁺ (2.82 and 18.74%) under elevated salt stress. OsNHX1 gene expression was downregulated (0.3 and 4.1-folds), whereas the gene expression of OsPIN1A, OsCATA, and OsAPX1 was upregulated by a 7-17-fold in bacterial-inoculated rice plants. It was concluded that the selected bacterial isolates, ALT11, ALT12, and ALT30, mitigated the adverse effects of salt stress on rice growth and can be used as climate smart agricultural tools in ecofriendly agricultural practices.

Keywords: antioxidants; genes expression; growth attributes; hormones; rice; salinity stress.