Abstract
Uptake of [3H]oleate by canine or rat cardiac myocytes is saturable, displays the countertransport phenomenon, and is inhibited by phloretin and trypsin. Cardiac myocytes contain a basic (pI approximately 9.1) 40-kD plasma membrane fatty acid binding protein (FABPPM) analogous to those recently isolated from liver, adipose tissue, and gut, unrelated to the 12-14-kD cytosolic FABP in these same tissues. An antibody to rat liver FABPPM selectively inhibits specific uptake of [3H]oleate by rat heart myocytes at 37 degrees C, but has no influence on nonspecific [3H]oleate uptake at 4 degrees C or on specific uptake of [3H]glucose. Uptake of long-chain free fatty acids by cardiac muscle cells, liver, and adipose tissue and absorption by gut epithelial cells is a facilitated process mediated by identical or closely related plasma membrane FABPs.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adipose Tissue / metabolism
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Animals
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Binding Sites, Antibody
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Binding, Competitive
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Biological Transport
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Carrier Proteins / immunology
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Carrier Proteins / isolation & purification
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Carrier Proteins / physiology*
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Dogs
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Fatty Acid-Binding Protein 7
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Fatty Acid-Binding Proteins
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Jejunum / metabolism
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Kinetics
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Liver / metabolism
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Membrane Proteins / immunology
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Membrane Proteins / isolation & purification
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Membrane Proteins / physiology*
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Molecular Weight
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Myocardium / immunology
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Myocardium / metabolism*
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Neoplasm Proteins*
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Nerve Tissue Proteins*
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Oleic Acid
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Oleic Acids / metabolism*
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Rats
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Rats, Inbred Strains
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Solutions
Substances
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Carrier Proteins
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Fabp7 protein, rat
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Fatty Acid-Binding Protein 7
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Fatty Acid-Binding Proteins
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Membrane Proteins
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Neoplasm Proteins
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Nerve Tissue Proteins
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Oleic Acids
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Solutions
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Oleic Acid