Comparison of the Prevalence of Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) among Staphylococcus aureus Isolates in a Burn Unit with Non-Burning Units

Hossein Sedaghat; Tahmineh Narimani; Bahram Nasr Esfahani; Sina Mobasherizadeh; Seyed Asghar Havaei

doi:10.18502/ijph.v50i1.5081

Comparison of the Prevalence of Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) among Staphylococcus aureus Isolates in a Burn Unit with Non-Burning Units

Iran J Public Health. 2021 Jan;50(1):146-151. doi: 10.18502/ijph.v50i1.5081.

Authors

Hossein Sedaghat¹, Tahmineh Narimani², Bahram Nasr Esfahani², Sina Mobasherizadeh³, Seyed Asghar Havaei^{4

2}

Affiliations

¹ Department of Microbiology, Faculty of Medicine, Infectious Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran.
² Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
³ Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
⁴ Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

Abstract

Background: Staphylococcus aureus (S. aureus) is one of the most important pathogens in burn infections colonized in the nose and increase the risk of infections.

Methods: Overall, 85 S. aureus isolates were isolated from clinical and nasal hospitalized patients and health care workers (HCWs) in a burn unit and non-burn units in Isfahan from June 2016 and September 2016. Genes encoding penicillin-binding protein 2a (mecA) and adhesive surface proteins, including fibronectin-binding proteins (fnbA,fnbB), fibrinogen binding protein (fib), laminin-binding protein(eno), collagen binding protein (cna), elastin binding protein (ebps), intracellular adhesion operon (icaA and icaD) were detected using PCR method.

Results: The rate of methicillin-resistant S. aureus (MRSA) among burn and non-burn isolates were 62% (18/29) and 25% (14/56), respectively. The most prevalent MSCRAMMs genes in burn units were eno (86%) and fib (66%). The most common gene pattern in burn center was icaA+fib+eno. The frequency of icaD, fib and ebpS was higher in clinical samples than nasal samples. No relation was found between the MSCRAMMs genes in the burn unit and non-burn units.

Conclusion: The high prevalence of MRSA in burn center can be a new challenge for clinicians. The higher frequency of icaD, fib and ebpS in clinical isolates than nasal isolates may reflect the important role of these genes in colonization and pathogenesis of S. aureus.

Keywords: Methicillin-resistant Staphylococcus aureus (MRSA); Proteins; Staphylococcus aureus; Surface proteins.