Fabrication and Characterization of Collagen/PVA Dual-Layer Membranes for Periodontal Bone Regeneration

Tian Zhou; Siwei Chen; Xinxin Ding; Zhihuan Hu; Lian Cen; Xiaomeng Zhang

doi:10.3389/fbioe.2021.630977

Fabrication and Characterization of Collagen/PVA Dual-Layer Membranes for Periodontal Bone Regeneration

Front Bioeng Biotechnol. 2021 Jun 9:9:630977. doi: 10.3389/fbioe.2021.630977. eCollection 2021.

Authors

Tian Zhou^{1

2}, Siwei Chen³, Xinxin Ding^{2

4}, Zhihuan Hu³, Lian Cen³, Xiaomeng Zhang^{2

4}

Affiliations

¹ Department of Oral Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
² National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, Shanghai, China.
³ Shanghai Key Laboratory of Multiphase Materials Chemical Engineering, State Key Laboratory of Chemical Engineering, Department of Product Engineering, School of Chemical Engineering, East China University of Science and Technology, Shanghai, China.
⁴ Department of Implant Dentistry, Shanghai Ninth Peoples' Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Abstract

Guided tissue regeneration (GTR) is a promising treatment for periodontal tissue defects, which generally uses a membrane to build a mechanical barrier from the gingival epithelium and hold space for the periodontal regeneration especially the tooth-supporting bone. However, existing membranes possess insufficient mechanical properties and limited bioactivity for periodontal bone regenerate. Herein, fish collagen and polyvinyl alcohol (Col/PVA) dual-layer membrane were developed via a combined freezing/thawing and layer coating method. This dual-layer membrane had a clear but contact boundary line between collagen and PVA layers, which were both hydrophilic. The dual membrane had an elongation at break of 193 ± 27% and would undergo an in vitro degradation duration of more than 17 days. Further cell experiments showed that compared with the PVA layer, the collagen layer not only presented good cytocompatibility with rat bone marrow-derived mesenchymal stem cells (BMSCs), but also promoted the osteogenic genes (RUNX2, ALP, OCN, and COL1) and protein (ALP) expression of BMSCs. Hence, the currently developed dual-layer membranes could be used as a stable barrier with a stable degradation rate and selectively favor the bone tissue to repopulate the periodontal defect. The membranes could meet the challenges encountered by GTR for superior defect repair, demonstrating great potential in clinical applications.

Keywords: GTR; PVA; collagen; dual-layer; periodontal.