A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients

STAR Protoc. 2021 Sep 17;2(3):100671. doi: 10.1016/j.xpro.2021.100671. Epub 2021 Jun 19.

Abstract

One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmission. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-based assay to detect antibodies against full-length SARS-CoV-2 spike protein (S protein) in patients with COVID-19. The assay is time-efficient and sensitive, being able to capture the wider repertoire of antibodies against the S protein. For complete details on the use and execution of this protocol, please refer to Goh et al. (2021).

Keywords: Antibody; Cell Biology; Flow Cytometry/Mass Cytometry; Health Sciences; Immunology; Molecular Biology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Neutralizing / blood*
  • Antibodies, Neutralizing / immunology
  • COVID-19 / blood
  • COVID-19 / diagnosis*
  • COVID-19 / immunology
  • COVID-19 / virology
  • COVID-19 Serological Testing / methods*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Flow Cytometry / methods*
  • Humans
  • SARS-CoV-2 / immunology*
  • Spike Glycoprotein, Coronavirus / immunology*

Substances

  • Antibodies, Neutralizing
  • Spike Glycoprotein, Coronavirus
  • spike protein, SARS-CoV-2