An integrated magneto-electrochemical device for the rapid profiling of tumour extracellular vesicles from blood plasma

Nat Biomed Eng. 2021 Jul;5(7):678-689. doi: 10.1038/s41551-021-00752-7. Epub 2021 Jun 28.


Assays for cancer diagnosis via the analysis of biomarkers on circulating extracellular vesicles (EVs) typically have lengthy sample workups, limited throughput or insufficient sensitivity, or do not use clinically validated biomarkers. Here we report the development and performance of a 96-well assay that integrates the enrichment of EVs by antibody-coated magnetic beads and the electrochemical detection, in less than one hour of total assay time, of EV-bound proteins after enzymatic amplification. By using the assay with a combination of antibodies for clinically relevant tumour biomarkers (EGFR, EpCAM, CD24 and GPA33) of colorectal cancer (CRC), we classified plasma samples from 102 patients with CRC and 40 non-CRC controls with accuracies of more than 96%, prospectively assessed a cohort of 90 patients, for whom the burden of tumour EVs was predictive of five-year disease-free survival, and longitudinally analysed plasma from 11 patients, for whom the EV burden declined after surgery and increased on relapse. Rapid assays for the detection of combinations of tumour biomarkers in plasma EVs may aid cancer detection and patient monitoring.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Immobilized / chemistry
  • Antibodies, Immobilized / immunology
  • Area Under Curve
  • Biomarkers, Tumor / blood
  • Biomarkers, Tumor / metabolism
  • Colorectal Neoplasms / diagnosis*
  • Colorectal Neoplasms / metabolism
  • Colorectal Neoplasms / mortality
  • Colorectal Neoplasms / surgery
  • Disease-Free Survival
  • Electrochemical Techniques / methods*
  • Epithelial Cell Adhesion Molecule / blood
  • Epithelial Cell Adhesion Molecule / metabolism
  • Extracellular Vesicles / immunology
  • Extracellular Vesicles / metabolism*
  • Female
  • Humans
  • Kaplan-Meier Estimate
  • Longitudinal Studies
  • Male
  • Middle Aged
  • Prognosis
  • ROC Curve
  • Recurrence
  • Young Adult


  • Antibodies, Immobilized
  • Biomarkers, Tumor
  • EPCAM protein, human
  • Epithelial Cell Adhesion Molecule