High-cell-density cultivation of the flagellate alga Poterioochromonas malhamensis for biomanufacturing the water-soluble β-1,3-glucan with multiple biological activities

Mingyang Ma; Yanhua Li; Jianping Chen; Fuchen Wang; Li Yuan; Yi Li; Baocai Zhang; Ding Ye; Danxiang Han; Hu Jin; Qiang Hu

doi:10.1016/j.biortech.2021.125447

High-cell-density cultivation of the flagellate alga Poterioochromonas malhamensis for biomanufacturing the water-soluble β-1,3-glucan with multiple biological activities

Bioresour Technol. 2021 Oct:337:125447. doi: 10.1016/j.biortech.2021.125447. Epub 2021 Jun 24.

Authors

Mingyang Ma¹, Yanhua Li¹, Jianping Chen¹, Fuchen Wang², Li Yuan¹, Yi Li³, Baocai Zhang⁴, Ding Ye³, Danxiang Han⁵, Hu Jin⁶, Qiang Hu⁷

Affiliations

¹ Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China.
² Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, PR China.
³ State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China.
⁴ State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100864, PR China.
⁵ Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China; Key Laboratory for Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China; The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100864, PR China.
⁶ Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China. Electronic address: jinhu@ihb.ac.cn.
⁷ Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China; Institute for Advanced Study, Shenzhen University, Shenzhen 518060, PR China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China; Key Laboratory for Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China; The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100864, PR China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. Electronic address: huqiang@szu.edu.cn.

PMID: 34186327
DOI: 10.1016/j.biortech.2021.125447

Abstract

The microalga Poterioochromonas malhamensis was found to be capable of accumulating the storage β-1,3-glucan in soluble form under heterotrophic conditions. In this study, the highest biomass yield of 32.8 g L^-1 was achieved by combining the utilization of ammonium chloride as the nitrogen source, simultaneous addition of vitamins B1 and B12 and maintenance of pH at 6.0. Sugar profiling and nuclear magnetic resonance analysis indicated that the P. malhamensis β-1,3-glucan was composed of glucose with the β-(1 → 3) main chain and the β-(1 → 6) side chain. Under the optimal cultivation conditions, the cellular β-1,3-glucan content was up to 55% of the cell dry weight. Moreover, the P. malhamensis β-1,3-glucan could significantly promote the fin regeneration and improve the in vivo antioxidative activity of zebrafish. This study underpins the feasibility of culturing P. malhamensis under heterotrophic conditions for producing the highly water-soluble bioactive β-1,3-glucans for food and pharmaceutical applications.

Keywords: Bioactivity; Heterotrophic cultivation; Poterioochromonas malhamensis; β-glucan.

MeSH terms

Animals
Glucans
Stramenopiles*
Water
Zebrafish
beta-Glucans*

Substances

Glucans
beta-Glucans
Water
beta-1,3-glucan