Acute gene inactivation in the adult mouse liver using the CRISPR-Cas9 technology

STAR Protoc. 2021 Jun 17;2(3):100611. doi: 10.1016/j.xpro.2021.100611. eCollection 2021 Sep 17.


Genetic manipulation in mice allows the discovery of gene function and biological mechanisms in vivo. The widely used Cre/LoxP system usually takes months to years especially when starting with the production of floxed alleles of a new gene of interest (GOI). Here, we describe a protocol using the CRISPR-Cas9 system to acutely inactivate the GOI in adult mice. This protocol enables hepatocyte-specific gene editing within 4 weeks in adult mice and avoids compensatory effects of traditional gene inactivation initiated during various developmental stages. For complete details on the use and execution of this protocol, please refer to Wang et al. (2020).

Keywords: CRISPR; Genetics; Model Organisms; Sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CRISPR-Cas Systems*
  • Gene Editing / methods
  • Gene Knockout Techniques
  • Liver / metabolism*
  • Mice
  • RNA, Guide, Kinetoplastida / genetics
  • Reproducibility of Results


  • RNA, Guide