Histological characterization and quantification of newborn cells in the adult rodent brain

Abstract

This protocol describes the identification and characterization of newborn cells generated in the rodent brain after injury through birthdating with the thymidine analog 5-bromo-2'-deoxyuridine, followed by immunohistochemical labeling of fixed tissue sections. We also describe a software-assisted approach for automated detection and quantification of cells in large three-dimensional tissue volumes acquired using confocal microscopy. This approach facilitates the identification of low-frequency events that may be difficult to capture using manual counting methods, including stereology based on random sampling. For complete details on the use and execution of this protocol, please refer to Ermine et al. (2020).

Keywords: Cell Biology; Microscopy; Model Organisms; Neuroscience.