Microalgae biofilm formation and antioxidant responses to stress induce by Lemna minor L., Chlorella vulgaris, and Aphanizomenon flos-aquae

Adamu Yunusa Ugya; Hadiza Abdullahi Ari; Xiuyi Hua

doi:10.1016/j.ecoenv.2021.112468

Microalgae biofilm formation and antioxidant responses to stress induce by Lemna minor L., Chlorella vulgaris, and Aphanizomenon flos-aquae

Ecotoxicol Environ Saf. 2021 Sep 15:221:112468. doi: 10.1016/j.ecoenv.2021.112468. Epub 2021 Jun 29.

Authors

Adamu Yunusa Ugya¹, Hadiza Abdullahi Ari², Xiuyi Hua³

Affiliations

¹ Key Lab of Groundwater Resources and Environment of Ministry of Education, Key Lab of Water Resources and Aquatic Environment of Jilin Province, College of New Energy and Environment, Jilin University, Changchun 130012, China; Department of Environmental Management, Kaduna State University, Kaduna, Nigeria.
² Key Lab of Groundwater Resources and Environment of Ministry of Education, Key Lab of Water Resources and Aquatic Environment of Jilin Province, College of New Energy and Environment, Jilin University, Changchun 130012, China; Faculty of Sciences, National Open University of Nigeria, Lagos, Nigeria.
³ Key Lab of Groundwater Resources and Environment of Ministry of Education, Key Lab of Water Resources and Aquatic Environment of Jilin Province, College of New Energy and Environment, Jilin University, Changchun 130012, China. Electronic address: huaxy@jlu.edu.cn.

PMID: 34198191
DOI: 10.1016/j.ecoenv.2021.112468

Abstract

The study shows how microalgae biofilm formation and antioxidant responses to the production of reactive oxygen species (ROS) is alter by the presences of Lemna minor L., Chlorella vulgaris, and Aphanizomenon flos-aquae. The study involves the cultivation of the biofilm of Chlorella vulgaris and Aphanizomenon flos-aquae in three bioreactors. The condition of growth for the biofilm formation was varied across the three bioreactors to enable the dominance Chlorella vulgaris and Aphanizomenon flos-aquae in one of the bioreactors. Lemna minor L. was also introduce into one of the bioreactors to determine its effect on the biofilm formation. The result obtained shows that C. vulgaris and A. flos-aquae dominate the biofilm, resulting in a high level of H₂O₂ and O₂^- (H₂O₂ was 0.122 ± 0.052 and 0.183 ± 0.108 mmol/L in C. vulgaris and A. flos-aquae, respectively, and O₂^- was 0.261 ± 0.039 and 0.251 ± 0.148 mmol/L in C. vulgaris and A. flos-aquae, respectively). The study also revealed that the presence of L. minor L. tend to reduce the oxidative stress to the biofilm leading to low production of ROS (H₂O₂ was 0.086 ± 0.027 and 0.089 ± 0.045 mmol/L in C. vulgaris and A. flos-aquae respectively, and O₂^- was 0.185 ± 0.044 and 0.161 ± 0.065 mmol/L in C. vulgaris and A. flos-aquae respectively). The variation in the ability of the biofilm of C. vulgaris and A. flos-aquae to respond via chlorophyll, carotenoid, flavonoid, anthocyanin, superoxide dismutase, peroxidase, catalase, glutathione reductase activities, antioxidant reducing power, phosphomolybdate activity, DPPH reduction activity, H₂O₂ scavenging activity, lipid content and organic carbon also supports the fact that the presence of biomass of microalgae and aquatic macrophytes tend to affect the process of microalgae biofilm formation and the ability of the biofilm to produce antioxidant. This high nutrient utilization leads to the production of biomass which can be used for biofuel production and other biotechnological products.

Keywords: Anthocyanin; Aquatic macrophytes; Catalase activity; ROS, Nanhu Lake; Total flavonoid.

MeSH terms

Antioxidants / pharmacology
Aphanizomenon / physiology*
Araceae / physiology*
Biofilms* / drug effects
Chlorella vulgaris / physiology*
Microalgae / physiology*
Oxidative Stress / drug effects
Reactive Oxygen Species

Substances

Antioxidants
Reactive Oxygen Species

Supplementary concepts

Aphanizomenon flos-aquae