Effects of a Fruit and Vegetable-Based Nutraceutical on Biomarkers of Inflammation and Oxidative Status in the Plasma of a Healthy Population: A Placebo-Controlled, Double-Blind, and Randomized Clinical Trial

Molecules. 2021 Jun 12;26(12):3604. doi: 10.3390/molecules26123604.


There is scientific evidence of the positive effect of polyphenols from plant foods on inflammation and oxidative status. The aim of the present study was to investigate whether treatment with a high-polyphenolic nutraceutical reduces the plasmatic concentration of certain oxidative and inflammatory biomarkers in a healthy population. One hundred and eight subjects were selected and stratified by sex in the intervention group (n = 53) and the placebo group (n = 55). Ninety-two subjects completed the study after two 16-week treatment periods separated by a four-week washout period. The results revealed statistically significant differences in subjects treated with the polyphenolic extract compared to the placebo: A decrease in homocysteine, oxidized low-density lipoprotein (OxLDL), TNF-α, sTNFR1, and C-reactive protein (CRP). The most significant decrease was observed for OxLDL (from 78.98 ± 24.48 to 69.52 ± 15.64; p < 0.05) and CRP (from 1.50 ± 0.33 to 1.39 ± 0.37; p < 0.05), both showing significant differences compared to the placebo (p < 0.001). Moreover, catecholamines increased after the administration of the product under investigation, especially in the case of dopamine (from 15.43 ± 2.66 to 19.61 ± 5.73; p < 0.05). Therefore, the consumption of a nutraceutical based on fruit and vegetables with a high polyphenol content seems to improve the parameters related to health benefits (oxidative and inflammatory biomarkers), including remarkable changes in the expression of catecholamines.

Keywords: biomarkers; fruit; nutraceutical; oxidative stress; polyphenol; vegetable.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Antioxidants / pharmacology
  • Biomarkers / blood
  • Catecholamines / metabolism
  • Dietary Supplements
  • Double-Blind Method
  • Female
  • Fruit / metabolism
  • Humans
  • Inflammation / drug therapy*
  • Lipoproteins, LDL / drug effects
  • Male
  • Oxidation-Reduction / drug effects
  • Oxidative Stress / drug effects
  • Placebos
  • Plant Extracts / pharmacology*
  • Polyphenols / pharmacology*
  • Vegetables / metabolism


  • Antioxidants
  • Biomarkers
  • Catecholamines
  • Lipoproteins, LDL
  • Placebos
  • Plant Extracts
  • Polyphenols
  • oxidized low density lipoprotein