Fluid flow-induced left-right asymmetric decay of Dand5 mRNA in the mouse embryo requires a Bicc1-Ccr4 RNA degradation complex

Nat Commun. 2021 Jul 1;12(1):4071. doi: 10.1038/s41467-021-24295-2.


Molecular left-right (L-R) asymmetry is established at the node of the mouse embryo as a result of the sensing of a leftward fluid flow by immotile cilia of perinodal crown cells and the consequent degradation of Dand5 mRNA on the left side. We here examined how the fluid flow induces Dand5 mRNA decay. We found that the first 200 nucleotides in the 3' untranslated region (3'-UTR) of Dand5 mRNA are necessary and sufficient for the left-sided decay and to mediate the response of a 3'-UTR reporter transgene to Ca2+, the cation channel Pkd2, the RNA-binding protein Bicc1 and their regulation by the flow direction. We show that Bicc1 preferentially recognizes GACR and YGAC sequences, which can explain the specific binding to a conserved GACGUGAC motif located in the proximal Dand5 3'-UTR. The Cnot3 component of the Ccr4-Not deadenylase complex interacts with Bicc1 and is also required for Dand5 mRNA decay at the node. These results suggest that Ca2+ currents induced by leftward fluid flow stimulate Bicc1 and Ccr4-Not to mediate Dand5 mRNA degradation specifically on the left side of the node.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Embryo, Mammalian / metabolism*
  • Gene Expression Regulation, Developmental
  • Intercellular Signaling Peptides and Proteins / genetics
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Mice
  • Mice, Knockout
  • RNA Stability / physiology*
  • RNA, Messenger / metabolism*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Receptors, CCR4 / genetics
  • Receptors, CCR4 / metabolism*
  • TRPP Cation Channels / metabolism
  • Transcription Factors


  • 3' Untranslated Regions
  • Bicc1 protein, mouse
  • CNOT3 protein, mouse
  • Ccr4 protein, mouse
  • Dte protein, mouse
  • Intercellular Signaling Peptides and Proteins
  • RNA, Messenger
  • RNA-Binding Proteins
  • Receptors, CCR4
  • TRPP Cation Channels
  • Transcription Factors
  • polycystic kidney disease 2 protein