CD38 Multi-Functionality in Oral Squamous Cell Carcinoma: Prognostic Implications, Immune Balance, and Immune Checkpoint

Zhuang Ding; Yijia He; Yong Fu; Nisha Zhu; Mengxiang Zhao; Yuxian Song; Xiaofeng Huang; Sheng Chen; Yan Yang; Caihong Zhang; Qingang Hu; Yanhong Ni; Liang Ding

doi:10.3389/fonc.2021.687430

CD38 Multi-Functionality in Oral Squamous Cell Carcinoma: Prognostic Implications, Immune Balance, and Immune Checkpoint

Front Oncol. 2021 Jun 15:11:687430. doi: 10.3389/fonc.2021.687430. eCollection 2021.

Authors

Zhuang Ding¹, Yijia He¹, Yong Fu¹, Nisha Zhu¹, Mengxiang Zhao¹, Yuxian Song¹, Xiaofeng Huang², Sheng Chen², Yan Yang², Caihong Zhang³, Qingang Hu¹, Yanhong Ni¹, Liang Ding¹

Affiliations

¹ Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.
² Department of Oral Pathology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.
³ Research Institute of Superconductor Electronics, School of Electronic Science and Engineering, Nanjing University, Nanjing, China.

Abstract

Background: CD38 belongs to the ribosyl cyclase family and is expressed on various hematological cells and involved in immunosuppression and tumor promotion. Although targeting CD38 antibodies has been approved for treatment of multiple myeloma, the function of CD38 in solid tumor, oral squamous cell carcinoma (OSCC) etc., has not been investigated.

Methods: This retrospective study included 92 OSCC samples and analyzed the spatial distribution of CD38 by immunohistochemistry (IHC). The values of diagnosis and prognosis of CD38 were evaluated. Additionally, 53 OSCC preoperative peripheral blood samples were used to be analyzed by flow cytometry. Tumor Immune Estimation Resource (TIMER) and cBioPortal databases were used to study CD38 level in various tumors and its correlation with tumor immune microenvironment in head and neck squamous cell carcinoma (HNSCC).

Results: CD38 ubiquitously presented in tumor cells (TCs), fibroblast-like cells (FLCs), and tumor-infiltrating lymphocytes (TILs). Patients with highly expressed CD38 in TCs (CD38^TCs) had higher TNM stage and risk of lymph node metastasis. Upregulation of CD38 in FLCs (CD38^FLCs) was significantly associated with poor WPOI. Escalated CD38 in TILs (CD38^TILs) led to higher Ki-67 level of tumor cells. Moreover, patients with enhanced CD38^TCs were susceptible to postoperative metastasis occurrence, and those with highly expressed CD38^TILs independently predicted shorter overall and disease-free survival. Strikingly, patients with highly expressed CD38^TILs, but not CD38^TCs and CD38^FLCs, had significantly lower CD3⁺CD4⁺ T cells and higher ratio of CD3^-CD16⁺CD56⁺NK cells. The imbalance of immune system is attributed to dysregulated immune checkpoint molecules (VISTA, PD-1, LAG-3, CTLA-4, TIGIT, GITR) as well as particular immune cell subsets, which were positively correlated with CD38 expression in HNSCC.

Conclusion: CD38 is a poor prognostic biomarker for OSCC patients and plays a vital role in governing immune microenvironment and circulating lymphocyte homeostasis. Co-expression between CD38 and immune checkpoint molecules provides new insight into immune checkpoint therapy.

Keywords: CD38; immune checkpoint; lymphocyte subsets; oral squamous cell carcinoma; prognosis.