Acoustic aggregation-induced separation for enhanced fluorescence detection of Alzheimer's biomarker

Yue Sun; Yong Luo; Tailin Xu; Guanzhi Cheng; Hong Cai; Xueji Zhang

doi:10.1016/j.talanta.2021.122517

Acoustic aggregation-induced separation for enhanced fluorescence detection of Alzheimer's biomarker

Talanta. 2021 Oct 1:233:122517. doi: 10.1016/j.talanta.2021.122517. Epub 2021 May 18.

Authors

Yue Sun¹, Yong Luo², Tailin Xu³, Guanzhi Cheng⁴, Hong Cai⁵, Xueji Zhang⁶

Affiliations

¹ School of Biomedical Engineering, Health Science Centre, Shenzhen University, Shenzhen, Guangdong, 518060, PR China; College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong, 518060, PR China.
² School of Biomedical Engineering, Health Science Centre, Shenzhen University, Shenzhen, Guangdong, 518060, PR China; School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, PR China.
³ School of Biomedical Engineering, Health Science Centre, Shenzhen University, Shenzhen, Guangdong, 518060, PR China; School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, PR China. Electronic address: xutailin@szu.edu.cn.
⁴ Institute of Railway Construction, China Academy of Railway Sciences Co., Ltd, Beijing, 100081, China.
⁵ Department of Chemistry, Hanshan Normal University, Chaozhou, China.
⁶ School of Biomedical Engineering, Health Science Centre, Shenzhen University, Shenzhen, Guangdong, 518060, PR China; College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong, 518060, PR China; School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, PR China. Electronic address: xuejizhang@szu.edu.cn.

PMID: 34215132
DOI: 10.1016/j.talanta.2021.122517

Abstract

On-chip microparticle-based separation is a significant activity in analytical and biomedical field. Here, we reported an acoustic aggregation-induced microparticle separation for on-chip fluorescence detection of Alzheimer biomarkers. miRNA-101, an Alzheimer-related biomarker, was used as a model target to validate the performance of the acoustic aggregation-induced separation assay. Under the ultrasound filed, the microparticles would move toward the centre of chip by simply adjusting the frequency and voltage. Such particle aggregation further resulted in fluorescence enhancement comparing with single microparticle. This approach integrated acoustic aggregation-induced microparticle separation with fluorescence enhancement, holding great potential application for the development of lab-on-a-chip based trace biomarkers detection in diagnosis field.

Keywords: Alzheimer's disease; Fluorescence enhancement; Separation; Ultrasound; microRNA.

MeSH terms

Acoustics
Alzheimer Disease* / diagnostic imaging
Biomarkers
Fluorescence
Humans
Lab-On-A-Chip Devices
MicroRNAs*

Substances

Biomarkers
MIRN101 microRNA, human
MicroRNAs