Ratiometric fluorescent detection and imaging of microRNA in living cells with manganese dioxide nanosheet-active DNAzyme

Yuan Zhang; Chunmeng Ma; Chen Ma; Yueci Xiang; Shuai Mu; Zhixiang Zheng; Xiaoyan Liu; Haixia Zhang

doi:10.1016/j.talanta.2021.122518

Ratiometric fluorescent detection and imaging of microRNA in living cells with manganese dioxide nanosheet-active DNAzyme

Talanta. 2021 Oct 1:233:122518. doi: 10.1016/j.talanta.2021.122518. Epub 2021 May 23.

Authors

Yuan Zhang¹, Chunmeng Ma², Chen Ma³, Yueci Xiang², Shuai Mu², Zhixiang Zheng³, Xiaoyan Liu², Haixia Zhang⁴

Affiliations

¹ State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China; Key Laboratory of Evidence Science Techniques Research and Application, Gansu Province, Gansu University of Political Science and Law, Lanzhou, 730070, China.
² State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China.
³ Key Laboratory of Evidence Science Techniques Research and Application, Gansu Province, Gansu University of Political Science and Law, Lanzhou, 730070, China.
⁴ State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China. Electronic address: zhanghx@lzu.edu.cn.

PMID: 34215133
DOI: 10.1016/j.talanta.2021.122518

Abstract

MicroRNAs (miRNAs) play an important role in multiple biological processes and can be used as biomarkers for clinical disease diagnosis, so their detection is of great importance. Here, manganese dioxide (MnO₂) nanosheet acts as carrier to deliver DNAzyme probes into cells through endocytosis, where intracellular glutathione (GSH) reduces the MnO₂ nanosheet to manganese ions (Mn²⁺) and releases the probes. The generated Mn²⁺ can be further used as an effective cofactor to activate the DNAzyme probe, and cleave the DNA strand into two fragments. Then, the miRNA-155 in the cells can hybridize with the cleaved fragment to cause the fluorescence signal change of the probe. The proposed proportional fluorescent method has been applied to the imaging of miRNA-155 in HeLa cells and HepG2 cells with the estimated detection limit (LOD) as 1.6 × 10^-12 M. The new method can provide great help for cancer diagnosis and biological research related to miRNA.

Keywords: DNAzyme; Fluorescence imaging; Manganese dioxide (MnO(2)) nanosheets; miRNA-155.

MeSH terms

DNA, Catalytic* / genetics
Fluorescent Dyes
HeLa Cells
Humans
Manganese Compounds
MicroRNAs* / genetics
Oxides

Substances

DNA, Catalytic
Fluorescent Dyes
MIRN155 microRNA, human
Manganese Compounds
MicroRNAs
Oxides
manganese dioxide