lncRNA MALAT1 regulated ATAD2 to facilitate retinoblastoma progression via miR-655-3p

Yuxin Zhao; Zhaoxia Wang; Meili Gao; Xuehong Wang; Hui Feng; Yuanyuan Cui; Xia Tian

doi:10.1515/med-2021-0290

lncRNA MALAT1 regulated ATAD2 to facilitate retinoblastoma progression via miR-655-3p

Open Med (Wars). 2021 Jun 24;16(1):931-943. doi: 10.1515/med-2021-0290. eCollection 2021.

Authors

Yuxin Zhao¹, Zhaoxia Wang², Meili Gao¹, Xuehong Wang¹, Hui Feng¹, Yuanyuan Cui¹, Xia Tian¹

Affiliations

¹ Department of Ophthalmology, Weihai Central Hospital, No. 3, Mishandongluxi, Wendeng District, Weihai, 264400, Shandong, China.
² Department of Pediatric, Weihai Central Hospital, Weihai, Shandong, China.

Abstract

Long noncoding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) was reported as an oncogene in many tumors including retinoblastoma (RB). This research mainly focused on the functions and mechanism of MALAT1 in RB. MALAT1 was upregulated in RB tissues and cells, and it served as a competing endogenous RNA (ceRNA) and inhibited miRNA-655-3p (miR-655-3p) expression, which eventually regulated the expression of miR-655-3p downstream target ATPase Family AAA Domain Containing 2 (ATAD2). The level of ATAD2 significantly increased, while that of miR-655-3p remarkably decreased in RB tissues and cells. MALAT1 depletion inhibited cell proliferation, metastasis, and epithelial-mesenchymal transition (EMT), but promoted apoptosis in vitro and blocked xenograft tumor growth in vivo. MALAT1 exerted its oncogenic functions in RB by regulating miR-655-3p/ATAD2 axis.

Keywords: ATAD2; MALAT1; miR-655-3p; retinoblastoma; tumor progression.