A fluorescent probe for STED microscopy to study NIP-specific B cells

Analyst. 2021 Jul 26;146(15):4744-4747. doi: 10.1039/d1an00601k.


We have developed a series of monovalent fluorophore-conjugated affinity probes based on the hapten 3-nitro-4-hydroxy-5-iodophenylacetyl (NIP), which is widely used as a model antigen to study B lymphocytes and the functional principles of B cell antigen receptors (BCRs). We successfully used them in flow-cytometry, confocal and super-resolution microscopy techniques.

MeSH terms

  • Antigens
  • B-Lymphocytes
  • Fluorescent Dyes*
  • Haptens
  • Microscopy*


  • Antigens
  • Fluorescent Dyes
  • Haptens