Chaperone-like protein DAY plays critical roles in photomorphogenesis

Nat Commun. 2021 Jul 7;12(1):4194. doi: 10.1038/s41467-021-24446-5.

Abstract

Photomorphogenesis, light-mediated development, is an essential feature of all terrestrial plants. While chloroplast development and brassinosteroid (BR) signaling are known players in photomorphogenesis, proteins that regulate both pathways have yet to be identified. Here we report that DE-ETIOLATION IN THE DARK AND YELLOWING IN THE LIGHT (DAY), a membrane protein containing DnaJ-like domain, plays a dual-role in photomorphogenesis by stabilizing the BR receptor, BRI1, as well as a key enzyme in chlorophyll biosynthesis, POR. DAY localizes to both the endomembrane and chloroplasts via its first transmembrane domain and chloroplast transit peptide, respectively, and interacts with BRI1 and POR in their respective subcellular compartments. Using genetic analysis, we show that DAY acts independently on BR signaling and chlorophyll biogenesis. Collectively, this work uncovers DAY as a factor that simultaneously regulates BR signaling and chloroplast development, revealing a key regulator of photomorphogenesis that acts across cell compartments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / growth & development
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Brassinosteroids / metabolism
  • Chlorophyll / biosynthesis
  • Chloroplasts / metabolism
  • Etiolation / physiology
  • Gene Expression Regulation, Plant / physiology
  • Gene Knockdown Techniques
  • HSP40 Heat-Shock Proteins / genetics
  • HSP40 Heat-Shock Proteins / isolation & purification
  • HSP40 Heat-Shock Proteins / metabolism*
  • Light
  • Membrane Proteins / genetics
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / metabolism*
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism
  • Morphogenesis / physiology*
  • Morphogenesis / radiation effects
  • Mutation
  • Plants, Genetically Modified
  • Protein Kinases / genetics
  • Protein Kinases / metabolism*
  • RNA-Seq
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Seedlings / growth & development
  • Signal Transduction / physiology

Substances

  • Arabidopsis Proteins
  • Brassinosteroids
  • HSP40 Heat-Shock Proteins
  • Membrane Proteins
  • Microtubule-Associated Proteins
  • POR protein, Arabidopsis
  • Recombinant Proteins
  • Chlorophyll
  • Protein Kinases
  • BRI1 protein, Arabidopsis